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KMID : 0387720120230020173
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Korean Journal of Blood Transfusion
2012 Volume.23 No. 2 p.173 ~ p.179
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Two Cases of Anti-Jka Whose Reactivity Was Disappeared after Enzyme Treatment
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Choi Moon-Suk
Cho Yong-Gon
Lee Jae-Hyeon
Kim Dal-Sik
Lee Hye-Soo
Choi Sam-Im
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Abstract
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We report on two cases of anti-Jka, whose reactivity disappeared on an antibody identification test using enzyme-treated red cells. One of two patients was a 72-year-old female with cirrhosis of the liver and colon cancer, and the other was a 55-year-old female with known MDS and incomplete Behcet’s disease. Results of an antibody identification test using a LISS/Coombs gel card (DiaMed AG) showed negative to one positive with red cells having the Jka antigen; however, all reactions using the enzyme-treated cells showed negative results, which was unexpected. The patients’ RBC phenotype was Jk(a?b＋). We obtained positive results in reactions of enzyme-treated Jka＋ cells and EDTA using a patient’s serum and proved that the cause of the negative reaction might be complement-related.
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KEYWORD
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Anti-Jka, Enzyme, EDTA
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